Synergistic function of rolB, rolC, ORF13 and ORF14 of TL-DNA of Agrobacterium rhizogenes in hairy root induction in Nicotiana tabacum

Abstract

Comparison of the frequency of rooting in the tobacco of the frequency of rooting in the tobacco leaf segments inoculated with Agrobacterium tumefaciens harboring various combinations of rolB, rolC, ORF13 and ORF14 of TL-DNA of Ri plasmid (pRiHRI) revealed that the genes differ in their function to stimulate adventitious root induction. A single gene rolB induced roots, while rolC, ORF13 and ORF14 independently promoted the root induction by the rolB gene. The effects of these genes on the rolB-mediated rooting were in the order of ORF13 > rolC≥ORF14.