Antioxidant, cholesterol lowering activity, and analysis of Caesalpinia bonducella seeds extract

Abstract

Tombili plant (Caesalpinia bonducella) belongs to the family of Fabaceae. The seed extract of tombili has been empirically used as a traditional medicine. The purpose of this research was to fractionate tombili seed extract and test their antioxidant and cholesterol lowering activities. Extraction was made into fractions using n-hexane, ethyl acetate, and methanol as a solvent. The chemical compound of the ethyl acetate fraction was analyzed using liquid chromatography-mass spectrometry (LC-MS/MS). Antioxidant activity was tested using the DPPH method. The highest antioxidant activity was shown in ethyl acetate fraction with an IC50 value of 86.153 μg/mL. The second was the methanol fraction with an IC50 value of 94.053 μg/mL, and the third was the n-hexane fraction with an IC50 value of 100.933 μg/mL. The cholesterol lowering activity analysis showed that all fractions could inhibit cholesterol. The highest anti-cholesterol activity shown in ethyl acetate fraction with the concentration of 600 μg/mL can inhibit 81.5% of the cholesterol activity. The LC-MS/MS analysis showed that the ethyl acetate fraction contained glucoside, homoplantaginin, and vernolic acid compounds.