Fluorescent nucleic acid systems for biosensor

Abstract

Modified nucleic acids have a wide range of applications in many areas of biochemistry. In particular, fluorescence-based nucleic acid systems have been studied extensively for their implementation in molecular biology as platforms for disease diagnosis. In this Account, we summarize the past two decades of our research efforts associated with fluorescent nucleic acid systems for bio-sensing. We discuss single-nucleotide polymorphismbased molecular systems and their fluorescence behaviors in duplex DNA; the effects of fluorophore labeling on the fluorescence signals of modified oligonucleotides; pH-responsive nucleic acidmodified fluorescence biosensors; the use of double-stranded DNA and three-way junctionbased molecular beacons (MBs) for the detection of biologically important mRNA and miRNA; quencher-free MBs and their applications in vivo; biologically significant G-quadruplex structures and i-motifbased fluorescent biosensors; the detection of trinucleotide repeats; and, finally, some other applications of modified nucleosides with unique properties.