Widespread Differential Expression of Coding Region and 3' UTR Sequences in Neurons and Other Tissues

Citations of this article
Mendeley users who have this article in their library.


Mature messenger RNAs (mRNAs) consist of coding sequence (CDS) and 5' and 3' UTRs, typically expected to show similar abundance within a given neuron. Examining mRNA from defined neurons, we unexpectedly show extremely common unbalanced expression of cognate 3' UTR and CDS sequences; many genes show high 3' UTR relative to CDS, others show high CDS to 3' UTR. In situ hybridization (19 of 19 genes) shows a broad range of 3' UTR-to-CDS expression ratios across neurons and tissues. Ratios may be spatially graded or change with developmental age but are consistent across animals. Further, for two genes examined, a 3' UTR-to-CDS ratio above a particular threshold in any given neuron correlated with reduced or undetectable protein expression. Our findings raise questions about the role of isolated 3' UTR sequences in regulation of protein expression and highlight the importance of separately examining 3' UTR and CDS sequences in gene expression analyses.




Kocabas, A., Duarte, T., Kumar, S., & Hynes, M. A. (2015). Widespread Differential Expression of Coding Region and 3’ UTR Sequences in Neurons and Other Tissues. Neuron, 88(6), 1149–1156. https://doi.org/10.1016/j.neuron.2015.10.048

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free