A Eco-friendly management of seed borne mycoflora of Mustard (Brassica sp.) through botanicals and determination of antifungal activity by SEM.

Abstract

The study was planned to investigate the efficiency of Plant extract of Moringa oleifera against, Datura stramonium, Azadirachta indica and Corton bonplandianum against some seed born fungi (Aspergillus sp., Rhizopus sp., Fusarium sp., Alternaria sp., Cochleobolus sp., Rhizoctonia sp.) isolated from oil seed (Jhumi) using standard microbiological procedures and to investigate effectiveness of plant extract on seed borne mycoflora by SEM study. The process well diffusion method is used to study the efficacy of Plant extract. The extracts were poured into the wells at different concentrations like 50mg/ml, 100mg/ml, 200mg/ml and 400mg/ml. After incubation zones of inhibition was carried out by agar plate observed. As the concentrations of extracts increased the activity also increased and thus the zones of inhibition too increase. Hence Moringa oleifera, Datura stramonium, Azadirachta indica and Corton bonplandianum can be used against various seed born fungal disease.