In silico cloning and sequence analysis of F3H gene in raphanus sativus L.

Abstract

Flavanone 3-hydroxylase is a key enzyme in the biosynthetic pathway of plant flavonoids. The complete cDNA sequence of Raphanus sativus L. F3H gene was in silico cloned using Brassica napus F3H-2 gene cDNA sequence (DQ513329.1) as a probe. Then the hydrophilicity, secondary structure, and advanced structure of F3H protein in R. sativus L. were analyzed using bioinformatics methods. The results showed that the cDNA was 1,437 bp, contains an open reading frame of 1,077 bp, encoding 358 amino acids. The protein coded by R. sativus L. F3H gene cDNA showed 98% similarity to B. napus F3H-2. Its secondary structure contains 38.55% alpha helix, 41.9% random coil and 4.93% beta turn. The homology analysis of 3D structure showed that the three-dimensional structure of the protein is a compact globular structure.