Angiogenesis, an important process for long term neurological recovery, could be induced by ischemic brain injury. In this chapter, we describe a system to deliver adeno-associated viral (AAV) vector- mediated gene therapy for ischemic stroke. This includes the methods to construct, produce, and purify an AAV vector expressing target gene and an approach to quantify the number of microvessels and capillary density with synchrotron radiation angiography (SRA) imaging.
CITATION STYLE
Tang, Y., Li, Y., Lin, X., Miao, P., Wang, Y., & Yang, G. Y. (2014). Stimulation of cerebral angiogenesis by gene delivery. Methods in Molecular Biology, 1135, 317–329. https://doi.org/10.1007/978-1-4939-0320-7_26
Mendeley helps you to discover research relevant for your work.