Super-resolution imaging has provided unprecedented insight in the molecular complexities of fundamental cell biological questions. For G protein-coupled receptors (GPCRs), its application to the study of receptor homomers and heteromers have unveiled the diversity of complexes these GPCRs can form at the plasma membrane at a structural and functional level. Here, we describe our methodological approach of photoactivated localization microscopy with photoactivatable dyes (PD-PALM) to visualize and quantify the spatial assembly of GPCR heteromers at the plasma membrane.
CITATION STYLE
Jonas, K. C., & Hanyaloglu, A. C. (2019). Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy. In Methods in Molecular Biology (Vol. 1947, pp. 337–348). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9121-1_19
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