Culture-independent detection of nontuberculous mycobacteria in clinical respiratory samples

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Abstract

Culture-based detection of nontuberculous Mycobacteria (NTM) in respiratory samples is time consuming and can be subject to overgrowth by nonmycobacterial bacteria. We describe a single-reaction TaqMan quantitative PCR assay for the direct detection of NTM species in clinical samples that is specific, sensitive, and robust.

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Scoleri, G. P., Choo, J. M., Leong, L. E. X., Goddard, T. R., Shephard, L., Burr, L. D., … Rogers, G. B. (2016). Culture-independent detection of nontuberculous mycobacteria in clinical respiratory samples. Journal of Clinical Microbiology, 54(9), 2395–2398. https://doi.org/10.1128/JCM.01410-16

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