Culture-based detection of nontuberculous Mycobacteria (NTM) in respiratory samples is time consuming and can be subject to overgrowth by nonmycobacterial bacteria. We describe a single-reaction TaqMan quantitative PCR assay for the direct detection of NTM species in clinical samples that is specific, sensitive, and robust.
CITATION STYLE
Scoleri, G. P., Choo, J. M., Leong, L. E. X., Goddard, T. R., Shephard, L., Burr, L. D., … Rogers, G. B. (2016). Culture-independent detection of nontuberculous mycobacteria in clinical respiratory samples. Journal of Clinical Microbiology, 54(9), 2395–2398. https://doi.org/10.1128/JCM.01410-16
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