Screening of M3 mutants for yellow vein mosaic virus resistance in greengram [Vigna radiata (L.) wilczek]

Abstract

Greengram [Vigna radiata (L.) Wilczek] is a cheap source of dietary protein for the poor, with high levels of folate and\riron compared with many other legumes. Mungbean yellow mosaic virus is also one of the destructive viral disease\raffecting yield potential of greengram both quantitatively and qualitatively. Induced mutations, have offered a single and\rshort alternative to conventional breeding including isolation, screening, selection and testing generation after generation.\rAn investigation was carried out in two mungbean genotypes viz., CO (Gg) 7 and NM 65 treated by two mutagens viz.,\rgamma rays at the doses of 300, 400 and 500 Gy and EMS treatments of 10, 20 and 30 mM. The trial was conducted in the\rresearch farm of Agricultural College and Research Institute, TNAU, Madurai during Kharif season 2013. The M2\rgeneration was raised as individual M1 plant basis. The treated and control populations of M2 generation were carefully\rscreened for pod shattering resistance. The yellow vein mosaic virus disease (YMV) incidence was recorded for all the\rplants in M3 generation for the selected 22 mutants. Based on field scoring, the mutants viz., M5, M18, M26, M46, M54, M58,\rM70, M71, M92 and M98 were identified as yellow vein mosaic virus resistant mutants. The mutants which showed field\rresistance were checked for YMV resistant gene using the gene specific primer ISSR811. Five mutants confirmed with\rmarker studies. These resistant mutants have been forwarded to next generation for further yield performance.