Fungal specific CD154+ T-cells have been described as a biomarker in invasive aspergillosis. The influence of sample storage on the detection of these cells was assessed. Six-hour delay prior to PBMC isolation is associated with an 18% decrease of cell viability and alterations of the cellular composition of the sample. This results in 87% reduction of CD154+ A. fumigatus specific cells due to reduced assay sensitivity and increased background values in unstimulated samples. If prompt cell measurement is not feasible, isolated PBMCs can be frozen (at -20°C and -80°C) and processed later with comparable assay reliability (mean value fresh vs. thawing: 0.126, 0.133; Pearson-Coefficient: 0.962).
CITATION STYLE
Wurster, S., Weis, P., Page, L., Lazariotou, M., Einsele, H., & Ullmann, A. J. (2017). Quantification of A. fumiga tu s-specific CD154+ T-cells-preanalytic considerations. In Medical Mycology (Vol. 55, pp. 223–227). Oxford University Press. https://doi.org/10.1093/mmy/myw054
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