Quantification of A. fumiga tu s-specific CD154+ T-cells-preanalytic considerations

9Citations
Citations of this article
6Readers
Mendeley users who have this article in their library.

Abstract

Fungal specific CD154+ T-cells have been described as a biomarker in invasive aspergillosis. The influence of sample storage on the detection of these cells was assessed. Six-hour delay prior to PBMC isolation is associated with an 18% decrease of cell viability and alterations of the cellular composition of the sample. This results in 87% reduction of CD154+ A. fumigatus specific cells due to reduced assay sensitivity and increased background values in unstimulated samples. If prompt cell measurement is not feasible, isolated PBMCs can be frozen (at -20°C and -80°C) and processed later with comparable assay reliability (mean value fresh vs. thawing: 0.126, 0.133; Pearson-Coefficient: 0.962).

Cite

CITATION STYLE

APA

Wurster, S., Weis, P., Page, L., Lazariotou, M., Einsele, H., & Ullmann, A. J. (2017). Quantification of A. fumiga tu s-specific CD154+ T-cells-preanalytic considerations. In Medical Mycology (Vol. 55, pp. 223–227). Oxford University Press. https://doi.org/10.1093/mmy/myw054

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free