Biofabrication of zinc oxide nanoparticles, characterization and cytotoxicity against pediatric leukemia cell lines

Abstract

In this work, we showed the synthesis of zinc oxide nanoparticles (ZnO NPs) using leaf extract of Punica granatum. Characterization results showed the synthesis of stable ZnO NPs with average size of 7 nm. Fourier transform infrared (FT-IR) and zeta potential results confirmed the capping of ZnO NPs with plant extract polyphenols. X-ray diffraction (XRD) and selected area electron diffraction (SAED) examinations showed the crystalline nature of prepared ZnO NPs. Further, the cytotoxic properties of the green synthesized ZnO NPs are examined against pediatric leukemia cells and the morphology of cells was studies under microscope. The results of cytotoxicity and microscopic images revealed the dose dependent cytotoxicity, indicating their prospects for the development of novel techniques and materials for pediatric leukemia cancer therapy.