A specific knockdown of transcription factor activities in Arabidopsis

Abstract

Transcription factors are pivotal for the control of development and the response of organisms to changes in the environment. Therefore, a detailed understanding of their functions is of central importance for biology. Over the years, different experimental methods have been developed to study the activities of transcription factors in plants. These methods include perturbation assays, where the activity of a given transcription factor is disrupted and subsequently, the resulting effects are monitored using molecular, genomic, or physiological approaches. Perturbation assays can also be used to distinguish primary roles of transcription factors of interest from secondary effects. Thus, molecular genetic experiments after perturbation can be advantageous or even necessary for the precise understanding of transcription factor function at a certain stage of plant development or in a single tissue or organ type. In this chapter, we describe several commonly used techniques to knock down transcription factor activities and provide detailed information on how those techniques are employed in the model plant Arabidopsis thaliana.