Oligonucleotide probes for phylogenetic detection of waterborne bacteria

Abstract

Waterborne diseases were globally occurred, and many people were suffered from nd often killed by them. In order to prevent outbreaks of waterborne diseases, rapid etection of pathogenic microbes in aquatic environment is the important strategy in addition to the construction of water supply and vaccination. The 16S ribosomal RNA (rRNA) gene is often used as a target gene for bacterial detection using hy bridization techniques. In this study, we aimed to design the oligonucleotide probes that could be used for the detection of waterborne bacteria with hybridization techniques because design of specific probes is important to assure for the precise detection of target bacteria. We then evaluated the specificities of designed probes by using an oligonucleotide microar-ray. In conclusion, we confirmed that seven designed oligonucleotide probes were suitable for the specific detection of waterborne bacteria. These probes appear to be used for 16S rRNA targeted hybridization techniques such as fluorescence in situ hybridization (FISH) and oligonucleotide microarray. © 2010 The Pharmaceutical Society of Japan.