Characterization of the glnK-amtB operon of Azotobacter vinelandii

Abstract

To determine whether in Azotobacher vinelandii the P(II) protein influences the regulation of nlf gene expression in response to fluxes in the ammonium supply, the gene encoding P(II) was isolated and characterized. Its deduced translation product was highly similar to P(II) proteins from other organisms, with the greatest degree of relatedness being exhibited to the Escherichia coli glnK gene product. A gene designated amtB was found downstream of and was cotranscribed with glnK as in E. coli. The AmtB protein is similar to functionally characterized ammonium transport proteins from a few other eukaryotes and one other prokaryote. glnK and amtB comprise an operon. Attempts to isolate a stable glnK mutant strain were unsuccessful, suggesting that glnK, like glnA, is an essential gene in A. vinelandii. amtB mutants were isolated, and although growth on limiting amounts of ammonium was similar in the mutant and wild-type strains, the mutants were unable to transport [14C]methylammonium.