Fabrication of Skeletal Muscle Tissue from C2C12 Myoblast Cell Towards the Use as Bio-Actuator

Abstract

Genome-wide expression profiles of antibody-producing CHO cells during suspension-adaptation into a serum-free medium have\r been characterized using CHO DNA microarrays. The transcriptome was analyzed with the RNA samples of cultures collected prior\r to and after adaptation. Over 500 genes and many major pathways were found to be profoundly affected by suspension adaptation.\r Among the down-regulated pathways were cell-cell adhesion, cell cycle, nucleotide synthesis, transcription, translation, vesiclular\r transport, microfilaments, and motor proteins. Among the up-regulated pathways were extracellular matrix, basement membrane,\r hypoxia signal pathway, antioxidant response elements, amino acid transporters as well as protein processing mechanisms in\r ER and Golgi. Most strikingly, the metabolic pathways were shifted to the de novo synthesis of fatty acids and sterols. The\r genes for fatty acid transport were significantly up-regulated and among them the mRNA of several key enzymes increased several\r hundred fold.