Fourier transform infrared spectroscopy (FT-IR) was used to scan whole bacterial cells as well as lipopolysaccharides (LPSs, endotoxins) isolated from them. Proteus mirabilis cells, with chemically defined LPSs, served as a model for the ATR FT-IR method. The paper focuses on three steps of infrared spectroscopy: (1) sample preparation, (2) IR scanning, and (3) multivariate analysis of IR data (principal component analysis, PCA).
CITATION STYLE
Żarnowiec, P., Czerwonka, G., & Kaca, W. (2017). Fourier transform infrared spectroscopy as a tool in analysis of proteus mirabilis endotoxins. In Methods in Molecular Biology (Vol. 1600, pp. 113–124). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6958-6_11
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