P-type ATPases can be expressed in several cell systems. The baculovirus expressions system uses an insect virus to enter and express proteins in Sf9 insect cells. This expression system is a lytic system in which the cells will die a few days after viral infection. Subsequently, the expressed proteins can be isolated. Insect cells are a perfect system to study P-type ATPases as they have little or no endogenous Na,K-ATPase activity and other ATPase activities can be inhibited easily. Here we describe in detail the expression and isolation of Na,K-ATPase and H,K-ATPase isoforms with the baculovirus expression system.
CITATION STYLE
Koenderink, J. B., & Swarts, H. G. P. (2016). Expression of Na,K-ATPase and H,K-ATPase isoforms with the baculovirus expression system. Methods in Molecular Biology, 1377, 71–78. https://doi.org/10.1007/978-1-4939-3179-8_8
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