Antioxidative capacity of vitamin E, vitamin C and their combination in cryopreserved Bhadavari bull semen

Abstract

Aim: The aim was to determine the antioxidative capacity of vitamin E, vitamin C and their combination (vitamin E+C) on standard semen parameters i.e., mortality, percent live sperms, percent abnormal sperms and acrosomal integrity in cryopreserved Bhadawari bull semen. Materials and Methods: Ejaculates collected from four Bhadawari bulls were evaluated and later pooled at 37°C. Pooled semen sample was divided into four equal parts viz. T-1, T-2, T-3 and C. The divided samples were diluted with a Trisbased extender containing the antioxidants vitamin E (5 mM) (T-1) vitamin C (5 mM) (T-2), vitamin E+C combination (5 mM+5 mM) (T-3) and extender containing no antioxidants (control, C). The diluted samples with different antioxidant concentration were cooled to 5°C, frozen in 0.25-ml French straws and stored in liquid nitrogen (-196°C). Frozen straws were thawed individually at 37°C for 20 s in a water bath for evaluation. Results: Semen extender supplementation with vitamin E (5 mM), vitamin C (5 mM) and vitamin E+C combination caused significant (p<0.01) increases in the seminal attributes while significant (p<0.01) decreases was observed in percent abnormal sperms in comparison to control group indicating that supplementation of antioxidant in form of vitamin and their combination improves the quality of post thaw semen. Significantly higher values of semen parameters were observed in the T-3 followed by T-1 and T-2 indicating the anti-oxidative capacity of different supplements utilized during the study. Conclusion: The results of present investigation concluded that combination of vitamin E+C has most profound effect in protecting sperms against reactive oxygen species production and cold shock when compared to vitamin E and vitamin C supplemented alone in the extender for semen dilution utilized for cryopreservation.