Engineering Yarrowia lipolytica for the synthesis of glutathione from organic by-products

4Citations
Citations of this article
9Readers
Mendeley users who have this article in their library.

Abstract

Tripeptide glutathione, which plays important roles in many cellular mechanisms, is also a biotechnology-oriented molecule with applications in medicine, food and cosmetic. Here, the engineering of the yeast Yarrowia lipolytica for the production of this metabolite at high titer values from various agro-industrial by-products is reported. The constitutive overexpression of the glutathione biosynthetic genes GSH1 and GSH2 encoding respectively γ-glutamylcysteine synthetase and glutathione synthetase, together with the INU1 gene from Kluyveromyces marxianus encoding inulinase yielded a glutathione titer value and a productivity of 644 nmol/mg protein and 510 µmol/gDCW, respectively. These values were obtained during bioreactor batch cultures in a medium exclusively comprising an extract of Jerusalem artichoke tuber, used as a source of inulin, and ammonium sulfate, used as a nitrogen source.

References Powered by Scopus

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

232933Citations
N/AReaders
Get full text

Analysis of relative gene expression data using real-time quantitative PCR and the 2<sup>-ΔΔC</sup>T method

149993Citations
N/AReaders
Get full text

Applications of inulin and oligofructose in health and nutrition

520Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Metabolic engineering of microorganisms for urban waste valorization

10Citations
N/AReaders
Get full text

Valorisation of Waste Bread for the Production of Yeast Biomass by Yarrowia lipolytica Bioreactor Fermentation

5Citations
N/AReaders
Get full text

Improvement of glutathione production by a metabolically engineered Yarrowia lipolytica strain using a small-scale optimization approach

4Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Do, D. T. H., & Fickers, P. (2020). Engineering Yarrowia lipolytica for the synthesis of glutathione from organic by-products. Microorganisms, 8(4). https://doi.org/10.3390/microorganisms8040611

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 2

33%

Researcher 2

33%

Professor / Associate Prof. 1

17%

Lecturer / Post doc 1

17%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 2

40%

Biochemistry, Genetics and Molecular Bi... 1

20%

Environmental Science 1

20%

Social Sciences 1

20%

Save time finding and organizing research with Mendeley

Sign up for free