Dynamic maps of UV damage formation and repair for the human genome

129Citations
Citations of this article
154Readers
Mendeley users who have this article in their library.

Abstract

Formation and repair of UV-induced DNA damage in human cells are affected by cellular context. To study factors influencing damage formation and repair genome-wide, we developed a highly sensitive single-nucleotide resolution damage mapping method [highsensitivity damage sequencing (HS-Damage-seq)]. Damage maps of both cyclobutane pyrimidine dimers (CPDs) and pyrimidinepyrimidone (6-4) photoproducts [(6-4)PPs] from UV-irradiated cellular and naked DNA revealed that the effect of transcription factor binding on bulky adducts formation varies, depending on the specific transcription factor, damage type, and strand. We also generated time-resolved UV damage maps of both CPDs and (6-4)PPs by HS-Damage-seq and compared them to the complementary repair maps of the human genome obtained by excision repair sequencing to gain insight into factors that affect UV-induced DNA damage and repair and ultimately UV carcinogenesis. The combination of the two methods revealed that, whereas UV-induced damage is virtually uniform throughout the genome, repair is affected by chromatin states, transcription, and transcription factor binding, in a manner that depends on the type of DNA damage.

References Powered by Scopus

Ultrafast and memory-efficient alignment of short DNA sequences to the human genome

16741Citations
N/AReaders
Get full text

WebLogo: A sequence logo generator

9558Citations
N/AReaders
Get full text

The MEME Suite

2543Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Formation of UV-induced DNA damage contributing to skin cancer development

285Citations
N/AReaders
Get full text

Simultaneous removal of antibiotics and antibiotic resistance genes from pharmaceutical wastewater using the combinations of up-flow anaerobic sludge bed, anoxic-oxic tank, and advanced oxidation technologies

210Citations
N/AReaders
Get full text

Differential damage and repair of DNA-adducts induced by anti-cancer drug cisplatin across mouse organs

136Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Hu, J., Adebali, O., Adar, S., & Sancar, A. (2017). Dynamic maps of UV damage formation and repair for the human genome. Proceedings of the National Academy of Sciences of the United States of America, 114(26), 6758–6763. https://doi.org/10.1073/pnas.1706522114

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 57

55%

Researcher 30

29%

Professor / Associate Prof. 13

13%

Lecturer / Post doc 4

4%

Readers' Discipline

Tooltip

Biochemistry, Genetics and Molecular Bi... 60

58%

Agricultural and Biological Sciences 31

30%

Chemistry 8

8%

Medicine and Dentistry 4

4%

Article Metrics

Tooltip
Mentions
News Mentions: 1

Save time finding and organizing research with Mendeley

Sign up for free