Radioimmunoassay of growth hormone-dependent insulinlike growth factor binding protein in human plasma

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Abstract

A specific radioimmunoassay has been established for a growth hormone-dependent insulinlike growth factor (IGF) binding protein (BP) from human plasma. Although the assay was directed against a 53-kD, acid-stable BP subunit, the main immunoreactive BP in the circulation had an apparent molecular mass of ~ 125 kD. Only higher primate species showed cross-reactivity, and IGF-I, IGF-II, and other peptides were without effect. Circulating BP levels in healthy subjects rose threefold from early childhood to puberty. In 65 adults aged 18 to 65, the mean level (± SD) was 6.12 ± 1.43 μg/ml, and declined with age. Strong growth hormone-dependence of BP was also seen; there was a 2.2-fold increase in active acromegaly and a 50-80% reduction in growth hormone deficiency. Poorly controlled diabetic subjects had BP levels 40% below normal, whereas in renal failure and third-term pregnancy a mild elevation was seen. Measurement of BP may provide a useful adjunct to IGF assays in growth disorders.

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Baxter, R. C., & Martin, J. L. (1986). Radioimmunoassay of growth hormone-dependent insulinlike growth factor binding protein in human plasma. Journal of Clinical Investigation, 78(6), 1504–1512. https://doi.org/10.1172/JCI112742

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