Human CD57+ germinal center-T cells are the major helpers for GC-B cells and induce class switch recombination

67Citations
Citations of this article
39Readers
Mendeley users who have this article in their library.

Abstract

Background: The function of CD57+ CD4+ T cells, constituting a major subset of germinal center T (GC-Th) cells in human lymphoid tissues, has been unclear. There have been contradictory reports regarding the B cell helping function of CD57+ GC-Th cells in production of immunoglobulin (Ig). Furthermore, the cytokine and co-stimulation requirement for their helper activity remains largely unknown. To clarify and gain more insight into their function in helping B cells, we systematically investigated the capacity of human tonsil CD57+ GC-Th cells in inducing B cell Ig synthesis. Results: We demonstrated that CD57+ GC-Th cells are highly efficient in helping B cell production of all four subsets of Ig (IgM, IgG, IgA and IgE) compared to other T-helper cells located in germinal centers or interfollicular areas. CD57+ GC-Th cells were particularly more efficient than other T cells in helping GC-B cells but not naïve B cells. CD57+ GC-Th cells induced the expression of activation-induced cytosine deaminase (AID) and class switch recombination in developing B cells. IgG1-3 and IgA1 were the major Ig isotypes induced by CD57+ GC-Th cells. CD40L, but not 1L-4, 1L-10 and IFN-.γ, was critical in CD57+ GC-Th cell-driven B cell production of Ig. However, 1L-10, when added exogenously, significantly enhanced the helper activity of CD57+ GC-Th cells, while TGF-β1 completely and IFN-γ. partially suppressed the CD57+ GC-Th cell-driven Ig production. Conclusions: CD57+CD4+ T cells in the germinal centers of human lymphoid tissues are the major T helper cell subset for GC-B cells in Ig synthesis. Their helper activity is consistent with their capacity to induce AID and class switch recombination, and can be regulated by CD40L, 1L-4, 1L-10 and TGF-β. © 2005 Kim et al; licensee BioMed Central Ltd.

Cite

CITATION STYLE

APA

Kim, J. R., Lim, H. W., Kang, S. G., Hillsamer, P., & Kim, C. H. (2005). Human CD57+ germinal center-T cells are the major helpers for GC-B cells and induce class switch recombination. BMC Immunology, 6. https://doi.org/10.1186/1471-2172-6-3

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free