Background: The function of CD57+ CD4+ T cells, constituting a major subset of germinal center T (GC-Th) cells in human lymphoid tissues, has been unclear. There have been contradictory reports regarding the B cell helping function of CD57+ GC-Th cells in production of immunoglobulin (Ig). Furthermore, the cytokine and co-stimulation requirement for their helper activity remains largely unknown. To clarify and gain more insight into their function in helping B cells, we systematically investigated the capacity of human tonsil CD57+ GC-Th cells in inducing B cell Ig synthesis. Results: We demonstrated that CD57+ GC-Th cells are highly efficient in helping B cell production of all four subsets of Ig (IgM, IgG, IgA and IgE) compared to other T-helper cells located in germinal centers or interfollicular areas. CD57+ GC-Th cells were particularly more efficient than other T cells in helping GC-B cells but not naïve B cells. CD57+ GC-Th cells induced the expression of activation-induced cytosine deaminase (AID) and class switch recombination in developing B cells. IgG1-3 and IgA1 were the major Ig isotypes induced by CD57+ GC-Th cells. CD40L, but not 1L-4, 1L-10 and IFN-.γ, was critical in CD57+ GC-Th cell-driven B cell production of Ig. However, 1L-10, when added exogenously, significantly enhanced the helper activity of CD57+ GC-Th cells, while TGF-β1 completely and IFN-γ. partially suppressed the CD57+ GC-Th cell-driven Ig production. Conclusions: CD57+CD4+ T cells in the germinal centers of human lymphoid tissues are the major T helper cell subset for GC-B cells in Ig synthesis. Their helper activity is consistent with their capacity to induce AID and class switch recombination, and can be regulated by CD40L, 1L-4, 1L-10 and TGF-β. © 2005 Kim et al; licensee BioMed Central Ltd.
Kim, J. R., Lim, H. W., Kang, S. G., Hillsamer, P., & Kim, C. H. (2005). Human CD57+ germinal center-T cells are the major helpers for GC-B cells and induce class switch recombination. BMC Immunology, 6. https://doi.org/10.1186/1471-2172-6-3