Production of L. pneumophila monospecies biofilms in a low-nutrient- concentration medium

Abstract

In aquatic environments such as water distribution systems, Legionella pneumophila persistence is correlated to the presence of a biofilm. The method described here permits the formation of a monospecies L. pneumophila biofilm in microplates, enabling the screening of multiple parameters. The culture medium used has a low nutrient concentration compared to the classical Buffered Yeast Extract culture medium. Hence, bacterial growth occurs in the sessile phase, allowing the formation of three-dimensional structures. © 2013 Springer Science+Business Media New York.