Gene silencing of a cubilin-related serine proteinase from the hard tick Haemaphysalis longicornis by RNA interference

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Abstract

RNA interference (RNAi) has been recently exploited to determine gene function by degrading specific mRNAs in several eukaryotic organisms. We constructed a double stranded RNA (dsRNA) from a previously cloned Haemaphysalis longicornis serine proteinase (HlSP) gene to test the importance of the function of the HlSP gene product during blood-feeding. Growth of unfed ticks treated with HlSP dsRNA was significantly inhibited compared to that of PBS-treated ticks. This inhibition was supported by the level of HlSP mRNA. HlSP may play a crucial role for blood-feeding in these ticks. This is the first report on gene silencing of a functional serine proteinase in hard ticks.

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Miyoshi, T., Tsuji, N., Islam, M. K., Kamio, T., & Fujisaki, K. (2004). Gene silencing of a cubilin-related serine proteinase from the hard tick Haemaphysalis longicornis by RNA interference. Journal of Veterinary Medical Science, 66(11), 1471–1473. https://doi.org/10.1292/jvms.66.1471

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