Enzyme inhibitory and antioxidant activities of different extracts from ruscus aculeatus l.

Abstract

The goal of this work was to evaluate in vitro antioxidant, anti-urease and anticho-linesterase activities of Ruscus aculeatus L. using a variety of extracts. Antioxidant activities of different extracts from plant were evaluated using DPPH, FRAP, ABTS/ TEAC and CUPRAC methods. In addition, the anti-urease and anticholinesterase activities of the different extracts were compared by Indophenol and Ellman tests, respectively. In the present study, Soxhlet chloroform extract showed stronger an-tioxidant (DPPH, FRAP, CUPRAC) and anticholinesterase activity than other ex-tracts. It was also found that the maceration ethanol extract showed the most potent anti-urease activity. The Soxhlet chloroform and maceration ethanol extracts from this species may be a natural resource candidate for the pharmaceutical and food industry due to the pharmacological effects (antioxidant, anticholinesterase and anti-urease effect).