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Multicolor single-particle reconstruction of protein complexes

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Abstract

Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reconstructs and coaligns multiple proteins from 2D super-resolution fluorescence images. To demonstrate our method, we generated multicolor 3D reconstructions of several proteins within the human centriole, which revealed their relative locations, dimensions and orientations.

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Sieben, C., Banterle, N., Douglass, K. M., Gönczy, P., & Manley, S. (2018). Multicolor single-particle reconstruction of protein complexes. Nature Methods, 15(10), 777–780. https://doi.org/10.1038/s41592-018-0140-x

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