New steroidal glycoside and flavonoid constituents from ophiopogon japonicus

Abstract

Chromatographic separations of a methanolic extract of Ophiopogon japonicus tubers resulted in the isolation of a new steroidal glycoside, (25R)-ruscogenin 1-O-(4-O-sulfo)-β-D-fucopyranoside (1), a new C-methylflavonol, 3,4'-dimethoxy-3',5,5',7-tetrahydroxy-8-methylflavone (2), and a known flavonol, 3,4'- dimethoxy-3',5,5',7-tetrahydroxyflavone (3). The C-methylflavonol was reported for the first time from the genus Ophiopogon. Compound 1 showed moderate cytotoxicity against SK-Mel-2 and KB cells with IC50 values of 24.3 and 28.8 μM, respectively. Compound 2 was active on SK-Mel-2 cells (IC50 20.3 μM).