ABO Reverse Grouping with “Laboratory Made” Red Blood Cells with Low Budget in a Small Transfusion Center in Algeria

Abstract

Objective: Reverse ABO group is required by Algerian Transfusion Guidelines in group identification. Immuno haematology laboratory with limited budget cannot afford commercialized reagent red cell. In this work, laboratory made test cells were used to determine their viability and interest in time gain and economically. Methods and materials: Double ABO group identification on blood donor and blood products using both forward ABO grouping with antisera test and reverse grouping with prepared 5% red blood cells test tube technique replaced slide technique. Results: 1095 identifications were performed. One discrepancy was observed with agglutinated B cells. Prepared red cell suspension presented hemolysis from day 5 of conservation and diminution of antigenic activity with low strength of agglutination with antisera test. Discussion: cold allo antibodies agglutinated B cell causing the discrepancy. Prepared red cell test can be used for 4days. Conclusion: In developing countries, reverse ABO grouping with laboratory made red cell, in blood group identification, is an economic alternative to ensure blood products and optimize patients outcomes.