Root carboxylate release is common in phosphorus-limited forest ecosystems in China: using leaf manganese concentration as a proxy

Abstract

Background and aims: Certain plant species release root carboxylates in response to phosphorus (P) limitation; however, the prevalence of root exudate release in species in P-limited forest ecosystems remains unexplored due to challenges in field assessment. Methods: Manganese (Mn) accumulation in mature leaves can indicate the presence of root carboxylate exudates in rhizosphere soil. To account for environmental factors such as soil pH, a negative reference species that does not release carboxylates is used for comparison. In this study, we assessed multiple forest stands across soil types and different levels of P availability in northern (Gansu) and southern (Guangxi) China. Leaf and soil samples were collected from 188 plant families representing various life forms, and leaf Mn concentration ([Mn]) was analyzed as a proxy for root carboxylate exudation patterns, using Dryopteridaceae as a negative reference. Results: The results supported our hypotheses that leaf [Mn] was higher in P-limited forests of southern China compared to P-richer forests of northern China, even though the soil [Mn] was higher in the forests of northern China. Additionally, we observed a higher prevalence of species with high leaf [Mn] across various plant families in Guangxi (82%) than in Gansu (42%). Conclusion: Our findings suggest a potential common strategy among plants in Guangxi forests, where root exudates are released in response to P limitation, possibly due to ineffective mycorrhizal symbiosis for nutrient acquisition. The diverse forest systems in China exhibit varying soil P availability, leading to the evolution of plant species with distinct P-acquisition strategies.