The close physiological similarity between the mouse and human has provided tools to understanding the biological function of particular genes in vivo by introduction or deletion of a gene of interest. Using a mouse as a model has provided a wealth of resources, knowledge, and technology, helping scientists to understand the biological functions, translocation, traffi cking, and interaction of a candidate gene with other intracellular molecules, transcriptional regulation, posttranslational modifi cation, and discovery of novel signaling pathways for a particular gene. Most importantly, the generation of the mouse model for a specifi c human disease has provided a powerful tool to understand the etiology of a disease and discovery of novel therapeutics. This chapter describes in detail the step-by-step generation of the transgenic mouse model, which can be helpful in guiding new investigators in developing successful models. For practical purposes, we will describe the generation of a mouse model using pituitary tumor transforming gene (PTTG) as the candidate gene of interest.
CITATION STYLE
Kakar, S. S., & Kakar, C. (2015). Generation of transgenic mouse model using pttg as an oncogene. Methods in Molecular Biology, 1267, 395–411. https://doi.org/10.1007/978-1-4939-2297-0_20
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