The quantitative reverse transcription polymerase chain reaction or real-time PCR has become a routine technique for the detection and comparison of amounts of specific mRNA transcripts, done by measuring amplified levels of specific cDNAs. In this chapter, we provide our real-time RT-PCR experimental procedure using SYBR Green I for the quantitative analysis of CCN family gene expression. Especially, we describe the extraction and purification steps for RNA derived from mesenchymal cells, such as chondrocytes and osteoblasts that produce a large amount of extracellular matrix in detail.
CITATION STYLE
Kawaki, H., Kubota, S., & Takigawa, M. (2017). Analysis of expression of CCN family genes in skeletal tissue-derived cells. In Methods in Molecular Biology (Vol. 1489, pp. 33–41). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6430-7_4
Mendeley helps you to discover research relevant for your work.