Evaluating macrophage migration inhibitory factor 1 expression as a prognostic biomarker in colon cancer

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Abstract

Objective: Several studies indicate that macrophage migration inhibitory factor 1 plays a role for tumor progression in colon cancer. We investigated whether determination of migration inhibitory factor 1 mRNA expression levels in lymph nodes of colon cancer patients could be used as a prognostic marker. Methods: Expression levels of migration inhibitory factor 1 and carcinoembryonic antigen mRNAs were assessed in primary tumors and regional lymph nodes of 123 colon cancer patients (stages I–IV), and in colon cancer- and immune cell lines using quantitative reverse transcriptase–polymerase chain reaction. Expression of migration inhibitory factor 1 protein was investigated by two-color immunohistochemistry and immunomorphometry. Results: Migration inhibitory factor 1 mRNA was expressed at 60 times higher levels in primary colon cancer tumors compared to normal colonic tissue (medians 8.2 and 0.2 mRNA copies/18S rRNA unit; p

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Olsson, L., Lindmark, G., Hammarström, M. L., Hammarström, S., & Sitohy, B. (2020). Evaluating macrophage migration inhibitory factor 1 expression as a prognostic biomarker in colon cancer. Tumor Biology, 42(6). https://doi.org/10.1177/1010428320924524

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