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Bacterial whole-cell biosensor for glutamine with applications for quantifying and visualizing glutamine in plants

Applied and Environmental Microbiology (2012) 78(2) 604-606
DOI: 10.1128/AEM.05261-11
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Abstract

A whole-cell biosensor for glutamine (GlnLux) was constructed by transforming an Escherichia coli glutamine (Gln) auxotroph with a constitutive lux reporter gene. Measurements of Gln in plant extracts using GlnLux correlated with quantification using high-performance liquid chromatography (Spearman's r=0.95). GlnLux permitted charge-coupled-device (CCD) imaging of Gln from whole plant organs. © 2012, American Society for Microbiology.

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Tessaro, M. J., Soliman, S. S. M., & Raizada, M. N. (2012). Bacterial whole-cell biosensor for glutamine with applications for quantifying and visualizing glutamine in plants. Applied and Environmental Microbiology, 78(2), 604–606. https://doi.org/10.1128/AEM.05261-11

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