Deoxyoligonucleotide microarrays for gene expression profiling in murine tooth germs

Abstract

The use of deoxyoligonucleotide microarrays facilitates rapid expression profiling of gene expression using samples of about 1 μg of total RNA. Here are described practical aspects of the procedures involved, including essential reagents. Analysis of results is discussed from a practical, experimental, point of view together with software required to carry out the required statistical analysis to isolate populations of differentially expressed genes. © 2012 Springer Science+Business Media, LLC.