IL-1β processing is one of the hallmarks of inflammasome activation and drives the initiation of the inflammatory response. For decades, Western blot or ELISA have been extensively used to study this inflammatory event. Here, we describe the use of a bioluminescence resonance energy transfer (BRET) biosensor to monitor IL-1β processing in real time and in living macrophages either using a plate reader or a microscope.
CITATION STYLE
Compan, V., & Pelegrín, P. (2016). Measuring IL-1β processing by bioluminescence sensors I: Using a bioluminescence resonance energy transfer biosensor. In Methods in Molecular Biology (Vol. 1417, pp. 89–95). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3566-6_5
Mendeley helps you to discover research relevant for your work.