The endocannabinoids anandamide and 2-arachidonoylglycerol are not only metabolized by serine hydrolases, such as fatty acid amide hydrolase, monoacylglycerol lipase, and α,β-hydrolases 6 and 12, but they also serve as substrates for cyclooxygenases and lipoxygenases. These enzymes oxygenate the 1 Z,4 Z-pentadiene system of the arachidonic acid backbone of endocannabinoids, thereby giving rise to an entirely new array of bioactive lipids. Hereby, a protocol is provided for the enzymatic synthesis, purification, and characterization of various oxygenated metabolites of anandamide generated by lipoxygenases, which enables the biological study and detection of these metabolites.
CITATION STYLE
van Zadelhoff, G., & van der Stelt, M. (2016). Oxygenation of anandamide by lipoxygenases. In Methods in Molecular Biology (Vol. 1412, pp. 217–225). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3539-0_22
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