High-capacity adenoviral vectors (HC-Ad), also known as "helper-dependent" (HD-Ad), "gutless", "gutted", or "third-generation" Ad vectors, are devoid of all viral coding sequences and have shown promising potential for a wide variety of different applications-from classic gene therapy to genetic vaccination and tumor treatment. However, compared to first-generation adenoviral vectors their production is more complex and requires specific in-depth knowledge. This chapter delivers a detailed protocol for the successful production of HC-Ad vectors to high titers. © 2014 Springer Science+Business Media, LLC.
CITATION STYLE
Kreppel, F. (2014). Production of high-capacity adenovirus vectors. Methods in Molecular Biology, 1089, 211–229. https://doi.org/10.1007/978-1-62703-679-5_15
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