Lipid affinity beads: From identifying new lipid binding proteins to assessing their binding properties

Abstract

Lipid affinity beads can be used to identify novel proteins with lipid binding capacity or to determine binding prerequisites of known lipid-binding proteins. Here we describe several applications for which this tool can be used and which considerations have to be taken into account. In addition to a precise protocol, several suggestions are made for experimental setups to facilitate identification of in vivo lipid binding targets. © 2013 Springer Science+Business Media, LLC.