Phospholipid-metabolizing enzymes are important participants in neutrophil signal transduction pathways. The methods discussed herein describe assays for assessing the activities of phospholipase A2 (PLA2), phospholipase C (PLC), phospholipase D (PLD), and phosphoinositide 3-OH-kinase in intact neutrophils. PLA2 activity is measured as the release of radiolabeled arachidonic acid. PLC activity is measured as the accumulation of inositol 1,4,5-trisphosphate (IP3), a water-soluble product, using a commercially available radioreceptor assay kit. PLD activity is measured as the appearance of its radiolabeled products, phosphatidic acid and phosphatidylethanol. PI3-K activity is measured as the appearance of its radiolabeled product, phosphatidylinositol-3,4,5-trisphosphate. © 2014 Springer Science+Business Media, LLC.
CITATION STYLE
Sergeant, S., & McPhail, L. C. (2014). Measurement of phospholipid metabolism in intact neutrophils. Methods in Molecular Biology, 1124, 89–105. https://doi.org/10.1007/978-1-62703-845-4_7
Mendeley helps you to discover research relevant for your work.