Exploring the hologenome concept in marine bivalvia: Haemolymph microbiota as a pertinent source of probiotics for aquaculture

79Citations
Citations of this article
130Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Haemolymph-associated microbiota of marine bivalves was explored for antibacterial activity against important aquaculture pathogens. A collection of 843 strains were cultured from the haemolymph of four bivalve species (Crassostrea gigas, Mytilus edulis, Pecten maximus and Tapes rhomboides) collected by deep-sea diving in the Glenan Archipelago (France). Cell-free culture supernatants were investigated for antibacterial activity using the well-diffusion assay. About 3% of haemolymph-associated cultivable bacteria displayed antibacterial activity toward Gram-negative pathogens. Among the active bacteria, Pseudoalteromonas strains exhibited the highest antibacterial activity. The cell-free culture supernatant of one of them, named hCg-51, was able to inhibit the growth of bacterial pathogens even after drastic dilution (1 : 1024). Hemocyte survival was not significantly altered in the presence of the haemolymph-associated strains assayed. Moreover, a dose-dependent beneficial effect on hemocyte survival rates was observed with the hCg-51 strain. These results suggest that haemolymph microbiota may participate in bivalve protection and therefore confer a health benefit on the host. As a result, the results highlight bivalve haemolymph microbiota as a promising novel source for aquaculture probiotics. This work also gives a first insight into the contribution of the haemolymph-associated microbiota as part of the bivalve 'hologenome'. Isolation of new Pseudoalteromonas strains exhibiting a high probiotics potential was the result of the first exploration of microbiota from bivalvia hemolymph for antibacterial activity and cytotoxic effect. © 2013 Federation of European Microbiological Societies.

Cite

CITATION STYLE

APA

Desriac, F., Le Chevalier, P., Brillet, B., Leguerinel, I., Thuillier, B., Paillard, C., & Fleury, Y. (2014, January). Exploring the hologenome concept in marine bivalvia: Haemolymph microbiota as a pertinent source of probiotics for aquaculture. FEMS Microbiology Letters. https://doi.org/10.1111/1574-6968.12308

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free