Observing brief and irregular behaviour of animals—the validity of short observation periods

Abstract

There are efficient sampling methods to accurately estimate behaviour with a moderate or long duration. For short behaviour, observing animals continuously is recommended although there is no recommended minimum observation time. In most studies, sampling method and observation time per day is determined by practical considerations. Thus, this study analysed the validity of behavioural observations in different observation periods using continuous sampling (CS) or time sampling (TS) based on biting behaviour. Tail-biting and ear-biting of weaned piglets in six pens were continuously observed for 12 h per day for 4 days to form a reference. Shorter observation periods of CS and TS were simulated by taking subsets of this reference. The amount of behaviour per hour of each observation period was compared to the reference and to other observation period of the same kind and length. Four different measurements were defined to calculate accuracy scores (AS; 0–1; higher values are better). Comparison to the reference shows better AS for observation periods with longer observation time in total (0.5 h of CS: 0.2; 6 h of CS: 0.6). Additionally, TS covers longer time periods without decreasing AS. However, focus on activity time results in an overestimation of irregular behaviour. Comparing AS among observation periods of the same kind and length show overall low agreement. This study indicated problems of different observation periods of CS and TS to accurately estimate behaviour. Therefore, validity of behavioural observations should be analysed in greater detail to determine optimal sampling methods.