As more genes are being identified through genomic techniques, the need to rapidly express recombinant proteins for functional studies has become increasingly acute. Transient expression of recombinant protein using COS-1, CV-1 and 293 cells is widely used to address this need. To improve the robustness of host cells for transient expression, the effect of over-expression of Protein Kinase Bα has been explored. In this report we demonstrate that over-expression of Protein Kinase Bα can improve transient recombinant protein expression 40% to >200% depending on the protein being expressed and the cell line used.
CITATION STYLE
Ettehadieh, E., Wong-Madden, S., Aldrich, T., Lane, K., & Morris, A. E. (2002). Over-expression of protein kinase enhances recombinant protein expression in transient systems. Cytotechnology, 38(1–3), 11–14. https://doi.org/10.1023/A:1021121107801
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