Kinetic parameters and relative turnovers of some important catabolic reactions in digesting sludge

Abstract

The kinetics of propionate degradation, acetate splitting, and hydrogen consumption in digesting sludge were investigated in a lab-scale digester. At natural steady-state conditions, the acetate-splitting systems in well-digested sludge were about half saturated. Propionate-degrading systems were saturated to only 10-15%, and hydrogen removal was less than 1% of the maximum possible rate. It was concluded that acetate splitting rather than 'methanogenesis from fatty acids' is the rate-limiting reaction in the anaerobic degradation of dissolved organic matter and that a methanogenic anaerobic ecosystem is stabilized by its large unused capacity of hydrogen consumption which is 'buffering' the partial pressure of dissolved hydrogen in the system at sufficiently low values to permit rapid fatty acid oxidation. A tentative scheme of the substrate flow in sludge digestion is presented. It suggests that acid formation coupled with hydrogen formation via pyridine dinucleotide oxidation yields the immediate substrates, namely acetate and hydrogen, for about 54% of the total methanogenesis.