Targeted electroporation in the CNS in Xenopus embryos

Abstract

Electroporation allows targeting of genetic materials (e.g., DNA, RNA, antisense morpholinos) to the tissue of interest with high spatial and temporal specificity. Here, we describe a highly efficient and reproducible electroporation strategy for targeting the central nervous system in Xenopus. This versatile approach can be combined with live imaging or other existing experimental procedures to aid the investigation of different research questions.