The effect of several nutritional and environmental parameters on Penicillium purpurogenum growth and sacharogenic amylase production was analyzed. High enzyme levels (68.2 U mg-1) were obtained with Khanna medium at initial pH 6.0, incubated at 30°C for 144 hours. The optimum pH and temperature activities were 5.0 and 65°C, respectively. The enzyme presented a half-life (t50) of 60 min, at 65°C. Only glucose was detected after 24 hours of reaction using soluble starch as substrate.
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Silva, T. M., de Oliveira, M., Somera, A. F., Jorge, J. A., Terenzi, H. F., Polizelide, M. de L. T. M., & Guimarães, L. H. S. (2011). Thermostable saccharogenic amylase produced under submerged fermentation by filamentous fungus Penicillium purpurogenum. Brazilian Journal of Microbiology, 42(3), 1136–1140. https://doi.org/10.1590/S1517-83822011000300035
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