Heterogeneous phenotypes of cancer cells enable them to adapt to various environments. The heterogeneity results from diversity of genome, transcriptome, and epigenome at a single-cell level. The C1 system can automatically perform single-cell capture and whole genome amplification (WGA) or whole transcription amplification (WTA) by MDA or Smart-Seq, respectively. Here, we describe the protocols for WGA and WTA from a single cell by using the C1 system and the protocols for sequence library preparation from amplified gDNA and cDNA. We also described about the computational analysis for single-cell data of cancer.
CITATION STYLE
Seki, M., Suzuki, A., Sereewattanawoot, S., & Suzuki, Y. (2019). Single-Cell DNA-Seq and RNA-Seq in Cancer Using the C1 System. In Advances in Experimental Medicine and Biology (Vol. 1129, pp. 27–50). Springer New York LLC. https://doi.org/10.1007/978-981-13-6037-4_3
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