We developed fixed-cell multi-well plate immunoassays that increase the throughput and ease of quantification for questions formerly assessed by immunoblot scanning. The assays make use of the now abundant antibodies designed to recognize receptor subtypes and posttranslationally modified signaling proteins. By optimizing permeabilization and fixation conditions, mainly based on specific cell types, the assay can be adapted to the study of many different antigens of importance to hormonal and neurotransmitter s ignaling scenarios.
CITATION STYLE
Watson, C. S., Jeng, Y. J., Bulayeva, N. N., Finnerty, C. C., Koong, L. Y., Zivadinovic, D., … Smith, T. D. (2014). Multi-well plate immunoassays for measuring signaling protein activations/deactivations and membrane vs. intracellular receptor levels. Methods in Molecular Biology, 1204, 123–133. https://doi.org/10.1007/978-1-4939-1346-6_11
Mendeley helps you to discover research relevant for your work.