Metabolomics analysis between wild-type and industrial strains of streptomyces avermitilis based on gas chromatography–mass spectrometry strategy

Abstract

Objective To conduct a metabolic analysis of the intracellular metabolites in Streptomyces avermitilis, so as to search for possible biomarkers and to discover the mechanism of higher avermectin production. Methods GC–MS analysis was used to obtain the fingerprint of wild-type and industrial S. avermitilis 9-39 strains. Results and conclusion multivariate statistical analysis demonstrated that D-cellobiose, D-galactose, D-glucopyranose, D-mannose, D-turanose, glutamine, L-serine, and maltose were mainly responsible for distinguishing the wild-type S. avermitilis and industrial strain 9-39, and most of them belong to glycometabolism. Thus, strengthened glycometabolism is the reason for high yield of avermectin in S. avermitilis.