Angiotensin I-converting enzyme inhibitory activity and antitumor activity of hatakeshimeji (Lyophyllum decastes Sing.)

Abstract

In the present study we have focused our attention mainly on both ACE inhibitory and antitumor activities of Hatakeshimeji (Lyophyllum decastes Sing.). A cold-water extract (CWE), a hot-water extract (HWE) and an ethanol extract (EE) showed significant ACE inhibitory activities, but their pronase hydrolysates did not show any ACE inhibitory activities, indicating that protein(s) and/or peptide(s) of Hatakeshimeji are responsible for ACE inhibitory activity. ACE inhibitory activities of CWEs of the other 8 edible mushrooms were also examined and compared with that of Hatakeshimeji. Hatakeshimeji was found to be the highest among the nine mushrooms tested. Furthermore, among the crude polysaccharides derived from HWEs of the nine mushrooms , Hatakeshimeji-derived polysaccharide was shown to possess the highest antitumor activity against solid tumor growth of Sarcoma 180 in mice. These results suggest that Hatakeshimeji may be an excellent functional food for preventing both cancer and hypertension.